Andrew N. Lane, PhD


James Graham Brown Endowed Chair of Structural Biology; Professor of Medicine, Chemistry and Biochemistry & Molecular Biology; Leader, Structural Biology Program, James Graham Brown Cancer Center; Director, NMR Facility, James Graham Brown Cancer Center; Associate Director (NMR) Center for Regulatory Environmental Analytical Metabolomics

Research Program
Structural Biology

Education
B.Sc., University College London, England, Biochemistry, 1972-1975
Ph.D., University College London, England, Biochemistry, 1975-1979

Research and Professional Experience

1979-1983
Post-doctoral researcher (with Professor Dr. K. Kirschner), Biophysical Chemistry, Biozentrum, University of Basel, Switzerland

1983-1986
EMBO Fellow (with Professor O. Jardetzky), Stanford Magnetic Resonance Laboratory, Stanford University

1986-1994
MRC Staff Scientist, National Institute for Medical Research, Mill Hill, London, UK

1992-2001
MRC Senior Staff Scientist, National Institute for Medical Research, Mill Hill, London, UK

1994-2001
Senior Staff Scientist, National Institiute for Medical Research, Mill Hill, London, UK

2001-present
Professor of Medicine, University of Louisville, Louisville, KY

Director, NMR Facility, James Graham Brown Cancer Center, University of Louisville

2002-present
Professor of Chemistry (joint), University of Louisville
Professor of Biochemistry & Molecular Biology, University of Louisville

Editorial Board Nucleic Acids Research

Editorial Board Journal of Structural and Functional Genomics

1997-2000
Committee British Biophysical Society

Selected Awards and Professional Honors

Honorary Research Fellow, Department of Biochemistry and Molecular Biology, University College London

James Graham Brown Endowed Chair of Structural Biology, University of Louisville

Program Leader, Structural Biology, James Graham Brown Cancer Center, University of Louisville

Associate Director, CREAM, University of Louisville

Member, NIGMS Metabolomics Network

2009
Mint Jubilee Scientist of the Year - Metabolomics Team

Research Interest

My research is divided into structural and biophysical analyses of macromolecules (DNA and proteins) and their interactions relevant to cancer biology, and stable isotope resolved metabolomics (SIRM) as applied to understanding cancer pathology, and as an approach for target discovery and validation.

Publications
Taylor IA, McIntosh PB, Pala P, Treiber MK, Howell S, Lane AN, Smerdon, SJ.  Characterization of the DNA-Binding Domains from the Yeast Cell-Cycle Transcription Factors Mbp1 and Swi4. Biochemistry  39:3943-54, 2000

McIntosh PB, Taylor IA, Frenkiel TA, Smerdon SJ, Lane AN.  The influence of DNA-binding on the backbone dynamics of the yeast cell-cycle protein Mbp1. J Biomolec NMR 16:183-96, 2000

Lane AN, Jenkins TC.  Thermodynamics of nucleic acids and their interactions with ligands. Q Rev Biophys 33, 255-306, 2000

Lane AN, Hays LM, Tsvetkova N, Feeney RE, Crowe LM, Crowe JH.  Comparison of the solution confor-mation and dynamics of antifreeze glycoproteins from Antarctic  fish.  Biophys J  78:3195-3207, 2000.

Gopal B, Papavinasasundaram KG, Dodson GG, Colston MJ, Major SA, Lane AN.  Spectroscopic and thermodynamic characterisation of the transcription antitermination factor NusE and its interaction with NusB from Mycobacterium tuberculosis. Biochemistry 40:920-8, 2001

Alexandrovich A, Czisch M, Frenkiel TA, Moolenaar GF, Goosen N, Sanderson MR, Lane AN.  Quaternary structure, thermodynamics and dynamics ofthe C-terminal domain of UvrB. J Biol Ster Dyn 19:219-36, 2001

Lane AN, Kelly G, Ramos A, Frenkiel TA.  Determining binding sites in protein-nucleic acid complexes by cross-saturation. J Biomolec NMR 21:127-39, 2001

Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN.  NMR structure of the DNA-binding domain of the cell cycle protein, Mbp1 from Saccharomyces cerevisiae. Biochemistry 42:1266-73, 2003

Gyi JI, Gao D, Conn GL, Trent JO, Brown T, Lane AN.  The solution structure of a DNA*RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications.  Nucleic Acids Res 31:2683-93, 2004

Fan TW, Lane AN, Chekmenev E, Wittebort RJ, Higashi RM.  Synthesis and physico-chemical properties of peptides in soil humic substances.  J Peptide Res 63(3):253-64, 2004.

Ramos A, Lane AN, Hollingworth D, Fan TW.  Secondary structure and stability of the selenocysteine insertion sequences (SECIS) for human thioredoxin reductase and glutathione peroxidase.  Nucleic Acids Res 32(5):1746-55, 2004

Fan TW, Lane AN, Higashi RM. An electrophoretic profiling method for thiol-rich phytochelations and metallothioneins.  Phytochem Anal 15(3):175-83, 2004

Fan T W-M, Higashi RM, Lane AN.  The promise of metabolomics in cancer molecular therapeutics. Current Opin. Molec. Ther
6(6):584-92, 2004 Review

Arumugam S, Gray RD, Lane AN.  1H, 15N and 13C assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa.  J Biomol NMR 31(3):265-6, 2005

Lane AN, Arumugam S.  Improving NMR sensitivity in room temperature and cooled probes with dipolar ions.  J Magn Reson 173(2):339-43, 2005

Booth J, Brown T, Vadhia SJ, Lack O, Cummins WJ, Trent JO, Lane AN.  Determining the origin of the stabilization of DNA by 5-aminopropynylation of pyrimidines.  Biochemistry 44(12):4710-9, 2005

Fan TW, Higashi RM, Lane AN. Integrating metabolomics and transcriptomics for probing SE anticancer mechanisms. Drug Metab Rev 38(4):707-32, 2006 Review

Telang S, Yalcin A, Clem AL, Bucala R, Lane AN, Eaton JW, Chesney J.  Ras transformation requires metabolic control by 6-phosphofructo-2-kinase.  Oncogene 25(55):7225-34, 2006

Telang S, Lane AN, Nelson KK, Arumugam S, Chesney J.  The oncoprotein H-RasV12 increases mitochondrial metabolism.  Mol Cancer 6:77, 2007

Kurtoglu M, Gao N, Shang J, Maher JC, Lehrman MA, Wangpaichitr M, Savaraj N, Lane AN, Lampidis TJ. Under normoxia, 2-deoxy-D-glucose elicits cell death in select tumor types not by inhibition of glycolysis but by interfering with N-linked glycosylation.  Mol Cancer Ther 6(11):3049-58, 2007

Lane AN, Chaires JB, Gray RD, Trent JO.  Stability and kinetics of G-quadruplex structures.  Nucleic Acids Res 36(17):5482-515, 2008

Deleeuw L, Tchernatynskaia AV, Lane AN.  Thermodynamics and specificity of the Mbp1-DNA interaction.  Biochemistry 47(24):6378-85, 2008

Lane AN, Fan TW, Higashi RM.  Stable isotope-assisted metabolomics in cancer research.  IUBMB Life 60(2):124-9, 2008 Review

Arumugam S, Gray RD, Lane AN. NMR structure note: alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa.  J Biomol NMR 40(3):213-7, 2008

Lane AN, Fan TW, Higashi RM.  Isotopomer-based metabolomic analysis by NMR and mass spectrometry.  Methods Cell Biol 84:541-88, 2008

Lane AN, Fan TW, Higashi RM.  Stable isotope-assisted metabolomics in cancer research.  IUBMB Life 60(2):124-9, 2008. Review

DeLeeuw L, Tchernatynskaia AV, Lane AN.  Thermodynamics and specificity of the Mbp1-DNA interaction.  Biochemistry 47(24):6378-85, 2008

Lane AN, Chaires JB, Gray RD, Trent JO.  Stability and kinetics of G-quadruplex structures.  Nucleic Acids Res 36(17):5482-515, 2008; PMC2553573. Review

Thornburg JM, Nelson KK, Clem BF, Lane AN, Arumugam S, Simmons A, Eaton JW, Telang S, Chesney J.  Targeting aspartate aminotransferase in breast cancer.  Breast Cancer Res 10(5):R84, 2008; PMC2614520

Fan TW, Kucia M, Jankowski K, Higashi RM, Ratajczak J, Ratajczak MZ, Lane AN.  Rhabdomyosarcoma cells show an energy producing anabolic metabolic phenotype compared with primary myocytes.  Mol Cancer 7:79, 2008; PMC2577687

Fan TW, Lane AN, Higashi RM, Bousamra M 2nd, Kloecker G, Miller DM.  Metabolic  profiling identifies lung tumor responsiveness to erlotinib.  Exp Mol Pathol 87(1):83-6, 2009; PMC2729693

Lane AN, Fan TW, Higashi RM, Tan J, Bousamra M, Miller DM.  Prospects for clinical cancer metabolomics using stable isotope tracers.  Exp Mol Pathol 86(3):165-73, 2009; PMC2685876

Fan TW, Lane AN, Higashi RM, Farag MA, Gao H, Bousamra M, Miller DM.  Altered regulation of metabolic pathways in human lung cancer discerned by (13)C stable isotope-resolved metabolomics (SIRM).  Mol Cancer 8:41, 2009; PMC2717907

Lane AN, Fan TW, Xie Z, Moseley HN, Higashi RM.  Isotopomer analysis of lipid biosynthesis by high resolution mass spectrometry and NMR.  Anal Chim Acta 651(2):201-8, 2009; PMC2757635

Chernatynskaya AV, Deleeuw L, Trent JO, Brown T, Lane AN.  Structural analysis of the DNA target site and its interaction with Mbp1.  Org Biomol Chem 7(23):4981-91, 2009

Yalcin A, Clem B, Makoni S, Clem A, Nelson K, Thornburg J, Siow D, Lane AN, Brock SE, Goswami U, Eaton JW, Telang S, Chesney J. Selective inhibition of choline kinase simultaneously attenuates MAPK and PI3K/AKT signaling.  Oncogene 29(1):139-49, 2010

Dailey MM, Miller MC, Bates PJ, Lane AN, Trent JO.  Resolution and characterization of the structural polymorphism of a single quadruplex-forming sequence.  Nucleic Acids Res 38:4877-88, 2010

 

Fan TW-M, Yuan P, Lane AN, Higashi RM, Wang Y, Hamidi A, Zhou R, Guitart-Navarro X, Chen G, Manji HK, Kaddurah-Daouk R.  Stable isotope resolved metabolomic analysis of lithium effects on glial-neuronal interactions.  Metabolomics 6:165-79, 2010

 

Arumugam S, Miller MC, Maliekal J, Trent JO, Lane AN.  NMR structure note: Solution structure of the RBD1,2 domains from human nucleolin.  J Biomolec NMR 47:79-83, 2010

 

Miller MC, Buscaglia R, Chaires JB, Lane AN, Trent JO.  Hydration determines the G-quadruplex stability and conformation of the human telomere 3’ sequence d(A(GGGTTA)3GGG)).  J Am Chem Soc 132:17105-107, 2010

 

Fan TW-M, Lane AN, Higashi RM, Yan J.  Stable isotope resolved metabolomics of lung cancer in a SCID mouse model.  Metabolomics 7:257-69, 2011

 

Fan TW-M, Lane AN.  NMR-based stable isotope resolved metabolomics.  In Systems Biochemistry.  J Biomolec NMR Sp Edn 49:267-80, 2011; DOI: 10.1007/s10858-011-9484-6

 

Lane AN, Fan TW-M, Bousamra II, M, Higashi RM, Yan J, Miller DM.  Stable Isotope-Resolved Metabolomics (SIRM) in cancer fesearch with clinical application to non-small cell lung cancer.  Omics 15:173-82, 2001; DOI: 10.1089/omi.2010.0088

 

Moseley HNB, Lane AN, Belshoff AC, Higashi RM, Fan TW-M.  Non-steady state modeling of UDP-GlcNAc biosynthesis enabled by stable isotope resolved metabolomics.  BMC Biology 9:37, 2011

 

Ye Z, Lane AN, Willing GA, Berson E.  Scaled up version of CBH1 from a cellulose mixture by ion exchange chromatography.  Biotech Progr, 2011; doi: 10.1002/btpr.696

 

Fan TW-M, Tan J, McKinney MM,  Lane AN.  Stable isotope resolved metabolomics analysis of ribonucleotide metabolism in lung cancer cells.  Metabolomics 2011; doi: 10.1007/s11306-011-0337-9

 

Chaires JB, Trent JO, Lane AN, Buscaglia R, Gray RD, Miller MC.  Chemical & Engineering News Letters To The Editor 89:6, 2011

 

Lane AN.  Bacterial repression:  The Lac and Trp Systems.  In: Online Encyclopedia of Biophysics, Springer Verlag. doi: 10.1007/978-3-642-16712-6, 2012

Contact Information

CTR Building
505 South Hancock Street
Louisville, KY 40202
(502) 852-3067


Fax: (502) 852-5679